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Salimetrics® Salivary α Amylase Enzyme Competitive Immunoassay Standard 96 Well Plate Assay (Elisa) Kit, supplied by Salimetrics LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Pre- and post-session <t>sCORT</t> for Session 1 (A) , Session 2 (B) , and Session 3 (C) (* denotes p < 0.05 by FRT, 5,000 iterations).
Salivary Cortisol Enzyme Competitive Immunoassay Standard 96 Well Plate Assay (Elisa) Kit, supplied by Salimetrics LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Pre- and post-session <t>sCORT</t> for Session 1 (A) , Session 2 (B) , and Session 3 (C) (* denotes p < 0.05 by FRT, 5,000 iterations).
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Pre- and post-session <t>sCORT</t> for Session 1 (A) , Session 2 (B) , and Session 3 (C) (* denotes p < 0.05 by FRT, 5,000 iterations).
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Pre- and post-session <t>sCORT</t> for Session 1 (A) , Session 2 (B) , and Session 3 (C) (* denotes p < 0.05 by FRT, 5,000 iterations).
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Validation of candidate MDA-MB-231-derived neoantigens. (A) For determination of cytotoxic activity of neoantigen-stimulated CD8+T cells, the bulk of CD8+T cells from donors 115 and 118 stimulated with the indicated neoantigen peptides were incubated overnight with carboxyfluorescein succinimidyl ester (CFSE)-labeled T2 cells pulsed with the neoantigen (red) or CFSE-labeled MDA-MB-231 cells (blue). Cell viability of target T-cells in these co-cultures was determined by staining with EthD-1 followed by flow cytometry analysis. Target T-cells gated on the CFSE-positive population that were positive for EthD-1 were considered dead cells. (B, C) CD8+T cells from a HLA-A*02:01-positive donor were stimulated as in (A) with peptide #22 and incubated overnight with peptide #22-negative/HLA-A*02:01-positive BT549 or peptide #22-positive/HLA-A*02:01-positive MDA-MB-231 cells with or without blocking anti-MHC-I antibodies. (B) Supernatants were collected and assayed <t>for</t> <t>IFN-γ</t> by <t>ELISA</t> and (B) cytotoxicity was determined as in (A). The data were analyzed by one-way ANOVA*p<0.05; **p<0.01; ***p<0.001. Graph shows mean±SEM. ANOVA, analysis of variance.
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Validation of candidate MDA-MB-231-derived neoantigens. (A) For determination of cytotoxic activity of neoantigen-stimulated CD8+T cells, the bulk of CD8+T cells from donors 115 and 118 stimulated with the indicated neoantigen peptides were incubated overnight with carboxyfluorescein succinimidyl ester (CFSE)-labeled T2 cells pulsed with the neoantigen (red) or CFSE-labeled MDA-MB-231 cells (blue). Cell viability of target T-cells in these co-cultures was determined by staining with EthD-1 followed by flow cytometry analysis. Target T-cells gated on the CFSE-positive population that were positive for EthD-1 were considered dead cells. (B, C) CD8+T cells from a HLA-A*02:01-positive donor were stimulated as in (A) with peptide #22 and incubated overnight with peptide #22-negative/HLA-A*02:01-positive BT549 or peptide #22-positive/HLA-A*02:01-positive MDA-MB-231 cells with or without blocking anti-MHC-I antibodies. (B) Supernatants were collected and assayed <t>for</t> <t>IFN-γ</t> by <t>ELISA</t> and (B) cytotoxicity was determined as in (A). The data were analyzed by one-way ANOVA*p<0.05; **p<0.01; ***p<0.001. Graph shows mean±SEM. ANOVA, analysis of variance.
Taqman ® Array Human Dna Methylation And Transcriptional Repression 96 Well Plate Standard Kit, supplied by EpiGentek, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Pre- and post-session sCORT for Session 1 (A) , Session 2 (B) , and Session 3 (C) (* denotes p < 0.05 by FRT, 5,000 iterations).

Journal: Frontiers in Neuroscience

Article Title: Group singing and its effect on cortisol, alpha amylase, oxytocin, and pain threshold in patients with Parkinson's disease

doi: 10.3389/fnins.2025.1569601

Figure Lengend Snippet: Pre- and post-session sCORT for Session 1 (A) , Session 2 (B) , and Session 3 (C) (* denotes p < 0.05 by FRT, 5,000 iterations).

Article Snippet: Cortisol samples were analyzed in-house using a Salimetrics© salivary cortisol enzyme competitive immunoassay standard 96-well plate assay (ELISA) kit.

Techniques:

Dot plot of delta pain threshold vs. delta sCORT. Session trendlines are generated using a generalized linear model.

Journal: Frontiers in Neuroscience

Article Title: Group singing and its effect on cortisol, alpha amylase, oxytocin, and pain threshold in patients with Parkinson's disease

doi: 10.3389/fnins.2025.1569601

Figure Lengend Snippet: Dot plot of delta pain threshold vs. delta sCORT. Session trendlines are generated using a generalized linear model.

Article Snippet: Cortisol samples were analyzed in-house using a Salimetrics© salivary cortisol enzyme competitive immunoassay standard 96-well plate assay (ELISA) kit.

Techniques: Generated

Validation of candidate MDA-MB-231-derived neoantigens. (A) For determination of cytotoxic activity of neoantigen-stimulated CD8+T cells, the bulk of CD8+T cells from donors 115 and 118 stimulated with the indicated neoantigen peptides were incubated overnight with carboxyfluorescein succinimidyl ester (CFSE)-labeled T2 cells pulsed with the neoantigen (red) or CFSE-labeled MDA-MB-231 cells (blue). Cell viability of target T-cells in these co-cultures was determined by staining with EthD-1 followed by flow cytometry analysis. Target T-cells gated on the CFSE-positive population that were positive for EthD-1 were considered dead cells. (B, C) CD8+T cells from a HLA-A*02:01-positive donor were stimulated as in (A) with peptide #22 and incubated overnight with peptide #22-negative/HLA-A*02:01-positive BT549 or peptide #22-positive/HLA-A*02:01-positive MDA-MB-231 cells with or without blocking anti-MHC-I antibodies. (B) Supernatants were collected and assayed for IFN-γ by ELISA and (B) cytotoxicity was determined as in (A). The data were analyzed by one-way ANOVA*p<0.05; **p<0.01; ***p<0.001. Graph shows mean±SEM. ANOVA, analysis of variance.

Journal: Journal for Immunotherapy of Cancer

Article Title: Personalized neoantigen viro-immunotherapy platform for triple-negative breast cancer

doi: 10.1136/jitc-2023-007336

Figure Lengend Snippet: Validation of candidate MDA-MB-231-derived neoantigens. (A) For determination of cytotoxic activity of neoantigen-stimulated CD8+T cells, the bulk of CD8+T cells from donors 115 and 118 stimulated with the indicated neoantigen peptides were incubated overnight with carboxyfluorescein succinimidyl ester (CFSE)-labeled T2 cells pulsed with the neoantigen (red) or CFSE-labeled MDA-MB-231 cells (blue). Cell viability of target T-cells in these co-cultures was determined by staining with EthD-1 followed by flow cytometry analysis. Target T-cells gated on the CFSE-positive population that were positive for EthD-1 were considered dead cells. (B, C) CD8+T cells from a HLA-A*02:01-positive donor were stimulated as in (A) with peptide #22 and incubated overnight with peptide #22-negative/HLA-A*02:01-positive BT549 or peptide #22-positive/HLA-A*02:01-positive MDA-MB-231 cells with or without blocking anti-MHC-I antibodies. (B) Supernatants were collected and assayed for IFN-γ by ELISA and (B) cytotoxicity was determined as in (A). The data were analyzed by one-way ANOVA*p<0.05; **p<0.01; ***p<0.001. Graph shows mean±SEM. ANOVA, analysis of variance.

Article Snippet: IFN-γ levels from the co-cultures were measured by ELISA Max Standard kit (BioLegend) in 96-well microtiter plates according to the manufacturer’s instructions.

Techniques: Biomarker Discovery, Derivative Assay, Immunopeptidomics, Activity Assay, Incubation, Labeling, Staining, Flow Cytometry, Blocking Assay, Enzyme-linked Immunosorbent Assay